BARLEY

MARIGOLD HYBRIDS

(Tagetes spp linn.)

I. Application and Amplification of General Seed Certification Standards

A. The General Seed Certification Standards are basic and together with the following specific standards

constitute the standards for certification of hybrids marigold seeds.

B. The General standards are amplified as follows to apply specifically to the hybrids of marigold:

1. Eligibility requirements for certification

(a) An inbred line to be eligible for certification shall be from a source such that its identity may

be assured and approved by the Certification Agency.

(b) Hybrid seed to be eligible for certification shall be the progeny of two approved inbred lines, one of

which shall be male sterile.

2. Classes and sources of seed

(a) An inbred line shall be a relatively true breeding strain resulting from self -pollination with selection and /

or developed through in-vitro culture.

(b) The Foundation class seed shall consist of an approved male sterile (apetalous) line to be used as a female parent

and an approved inbred line to be used as a male parent for the purpose of producing hybrid seed.

pollen and is maintained by the monogenic cominant hetrozygous strain and is used as maintainer in propagation

blocks.

(d) The Certified class seed shall be the hybrid seed to be planted for any use except seed production.

*(Applicable if male sterile line is used)

II. Land requirements

A seed crop of hybrid marigold shall not be eligible for certification if planted on land on which the same

kind of crop was grown in the previous year unless the crop (s) grown in the previous year was / were of the

same variety and of an equivalent or higher class of certified seed and was/ were certified.

III. Field Inspection

A minimum of three inspections shall be made as follows:

1. the first inspection shall be made at the stage of 6 - 7 pairs of leaves in order to determine isolation,

outcrosses, volunteer plants, planting ratio, (demarcation between female and male block if planted separately)

and other relevant factors .

2. the second and third inspection, shall be made during flowering to check isolation, pollen shedding heads in seed

parent, offtypes and other relevant factors;

3. the fourth inspection shall made at maturity prior to harvesting to verify designated diseases, true nature of

plant and capitulum and other relevant factors.

IV. Field Standards

A. General requirements

1. Isolation

Seed fields of marigold hybrid shall be isolated from the contaminants shown in the column 1 of the Table below

by the distances specified in columns 2 and 3 of the said Table:

Contaminants Minimum distance (meters)

Foundation Certified

1 2 3

Fields of other varieties including commercial hybrids

of the same cultivar. 600 300

Fields of the same hybrid (code designation)

not conforming to varietal purity requirements

for certification 600 300

Between blocks of the parental lines in case seed

parent and pollinator are planted in separate blocks - 5

B. Specific requirements

Factor Maximum permitted (%)*

Foundation Certified

1 2 3

*Offtypes in seed parent at and after flowering 0.050 0.10

Offtypes in pollinator at flowering 0.050 0.10

Pollen shedding heads in seed parent at flowering 0.050 0.10

*Plants affected by designated disease at final inspection 0.10 0.20

* Designated diseases shall be

Leaf spot (Alternana tagetica shome & Mustefee)

Flower bud rot (A. alternata (Fr.) Keissler n& A.dianthu Stevans & Hall)

Collar rot (Rhizoctonia solani Kuhn)

V. Seed standards

Factor Standards for each classes

Foundation Certified

1 2. 3.

Pure seed (minimum) 97.0% 97.0%

Inert matter (maximum) 3.0% 3.0%

Other crop seeds (maximum) 10/kg 20/kg

Weed seeds (maximum) 5/kg 10/kg

Germination (minimum) 70% 70%

Moisture (maximum) 9.0% 9.0%

For vapour-proof containers (maximum) 7.0% 7.0%