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HYBRID COTTON
1.Application and Amplification of General Seed Certification Standards
The General Seed Certification Standards are basic and, together with the following specific standards constitute the standards for certification of the seed of cotton hybrids.
B. The General Standards are amplified as follows to apply specifically to the hybrids of cotton:
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1. Eligibility requirements for certification if male sterile lines are used for producing hybrid seed:
a) An inbred line to be eligible for certification shall be from a source such that its identity may be assured and approved by the Certification Agency.
b) Hybrid seed to be eligible for certification shall be the progeny of two approved inbred lines, one of which shall be male sterile.
2.Classes and Sources of seed
(a) An inbred line shall be a relatively true breeding strain resulting from self-pollination with selection.
(b) The foundation class seed shall consist of an approved male sterile line to be used as a female parent and an approved inbred line to be used as a male parent for the purpose of producing hybrid seed.
(c) A male sterile line shall be a strain (A) carrying cytoplasmic genetic male sterility, which sheds no viable pollen and is maintained by the normal sister strain (B) which is used as pollinator.
(d) The certified class seed shall be the hybrid seed to be planted for any use except seed production.
II. Land Requirements
Land to be sued for seed production of hybrid cotton shall be free of volunteer plants.
III. Field Inspection
A. Foundation Seed Production
A minimum of three inspections shall be made, the first and second during flowering and the third at maturity and prior to harvesting.
B. Certified Seed Production
A minimum of four inspections shall be made as follows:
(1) the first inspection shall be made before flowering to verify isolation, volunteer plants, out crosses and other relevant factors;
(2) the second and third inspections shall be made during flowering to verify isolation, off types and other relevant factors. In case male sterile line is used for producing hybrid seed, the number of pollen shedding plants in female parent shall also be verified;
(3) the fourth inspection shall be made during picking of cotton in female parent in order to determine that selfed bolls are eliminated and only cotton from crossed bolls is picked. This inspection is not required for the fields if a male sterile line is used.
IV. Field Standards
A.General requirements
1.Isolation
Seed fields shall be isolated from the contaminants shown in column 1 of the Table below by the distances specified in columns 2 and 3 of the said Table:
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Contaminants
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Minimum distance(meters)
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Foundation
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Certified
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| Fields of other varieties of same species including commercial hybrid of the same variety |
50
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30
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| Fields of the same variety (code designation) not conforming to varietal purity requirements for certification |
50
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30
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| Fields of other varieties of different Spp. (different ploidy levels) |
5
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5
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| Between blocks of the parental lines Of the same hybrid |
--
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5
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B. Specific requirements
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Factor
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Maximum permitted(%)*
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Foundation
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Certified
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| Offtype |
0.10
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0.50**
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| ***Pollen shedding plants in seed parent |
0.05
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0.10
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* Maximum permitted at any inspection at and after flowering.
** Standard shall be met separately for seed parent and pollinator.
*** It shall be applicable in case male sterile line is used for the production of hybrid seed.
V. Seed Standards
(a) All certified seed lots which have been produced by adopting emasculation shall be subjected to grow-out test and shall conform to the following minimum genetic purity requirements:
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Class
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Genetic purity (%)*(minimum)
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| Certified |
90.0
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* During grow-out test, the offtype plants (other than selfed plants) such as segregants, out crosses and plants of other varieties should not exceed more than 1.50% out of10.0% plants earmarked for selfed plants.
(b) The minimum population size of 400 plants shall be maintained in two replicates of 200 each or four of 100 each throughout the test and each plant shall be examined individually. The reject number shall be as follows:
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Class
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Genetic purity (%) (minimum)
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Reject Number
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| Certified |
90.0(10 in 100)
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44
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Seed Standards
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Factor
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Standards for each class
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Foundation
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Certified
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98.0%
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98.0%
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2.0%
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2.0%
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5/kg
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10/kg
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5/kg
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10/kg
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65%
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65%
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10.0%
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10.0%
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6.0%
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6.0%
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VI. Ginning
Ginning of seed cotton shall be done on the gins approved by the Certification Agency.
